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Lookup NU author(s): Dr Arthur Mckie,
Professor Hing Leung
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DNA fingerprinting can be used to detect genetic rearrangements in cancer that may be associated with activation of oncogenes and inactivation of tumour suppressor genes. We have developed a fingerprinting strategy based on polymerase chain reaction (PCR) amplification of genomic DNA with primers specific for the Alu repeat sequences, which are highly abundant in the human genome. This has been applied to DNA from pancreatic cancer and paired normal samples to isolate and identify fragments of genomic DNA rearranged in the malignant cells. These fragments have been sequenced and used as probes to isolate hybridising clones from gridded bacteriophage PI, phage artificial chromosome, and cosmid libraries for fluorescent in situ hybridisation mapping and the identification of expressed sequences. Further characterisation has identified a putative novel gene (ART1) that is up-regulated specifically in pancreatic cancer as well as another sequence with similarity to genes involved in differentiation (POU domains). In conclusion, we suggest that Alu-PCR fingerprinting may be a useful technique for the identification of genes involved in tumourigenesis.
Author(s): McKie AB, Iwamura T, Leung HY, Hollingsworth MA, Lemoine NR
Publication type: Article
Publication status: Published
Journal: Genes, Chromosomes & Cancer
Print publication date: 07/12/1998
ISSN (print): 1045-2257
ISSN (electronic): 1098-2264
Publisher: John Wiley & Sons, Inc.
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