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Lookup NU author(s): Dr Stephen Addinall
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In vitro polymerization of the essential bacterial cell division protein FtsZ, in the presence of GTP, is rapid and transient due to its efficient binding and hydrolysis of GTP. In contrast, the in vivo polymeric FtsZ structure which drives cell division - the Z-ring - is present in cells for extended periods of time whilst undergoing constant turnover of FtsZ. It is demonstrated that dynamic polymerization of Escherichia coli FtsZ in vitro is sensitive to the ratio of GTP to GDP concentration. Increase of GDP concentration in the presence of a constant GTP concentration reduces both the duration of FtsZ polymerization and the initial light-scattering maximum which occurs upon addition of GTP. It is also demonstrated that by use of a GTP-regeneration system, polymers of FtsZ can be maintained in a steady state for up to 85 min, while preserving their dynamic properties. The authors therefore present the use of a GTP-regeneration system for FtsZ polymerization as an assay more representative of the in vivo situation, where FtsZ polymers are subject to a constant, relatively high GTP to GDP ratio.
Author(s): Addinall SG; Small E
Publication type: Article
Publication status: Published
ISSN (print): 1350-0872
ISSN (electronic): 1465-2080
Publisher: Society for General Microbiology
Notes: Small, Elaine
Addinall, Stephen G
Research Support, Non-U.S. Gov't
Microbiology (Reading, England)
Microbiology. 2003 Aug;149(Pt 8):2235-42.
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