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The Enterococcus hirae copper chaperone CopZ delivers copper(I) to the CopY repressor

Lookup NU author(s): Dr Dr MD Harrison Harrison

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Abstract

Expression of the cop operon which effects copper homeostasis in Enterococcus hirae is controlled by the copper responsive repressor CopY. Purified Zn(II)CopY binds to a synthetic cop promoter fragment in vitro. Here we show that the 8 kDa protein CopZ acts as a copper chaperone by specifically delivering copper(I) to Zn(II)CopY and releasing CopY from the DNA. As shown by gel filtration and luminescence spectroscopy, two copper(I) are thereby quantitatively transferred from Cu(I)CopZ to Zn(II)CopY, with displacement of the zinc(II) and transfer of copper from a non-luminescent, exposed, binding site in CopZ to a luminescent, solvent shielded, binding site in CopY.

Publication metadata

Author(s): Cobine P, Wickramasinghe WA, Harrison MD, Weber T, Solioz M, Dameron CT

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 1999

Volume: 445

Issue: 1

Pages: 27-30

Print publication date: 19/02/1999

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468

Publisher: Elsevier

URL: http://dx.doi.org/10.1016/S0014-5793(99)00091-5

DOI: 10.1016/S0014-5793(99)00091-5

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