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Lookup NU author(s): Dr Dr MD Harrison Harrison
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Expression of the cop operon which effects copper homeostasis in Enterococcus hirae is controlled by the copper responsive repressor CopY. Purified Zn(II)CopY binds to a synthetic cop promoter fragment in vitro. Here we show that the 8 kDa protein CopZ acts as a copper chaperone by specifically delivering copper(I) to Zn(II)CopY and releasing CopY from the DNA. As shown by gel filtration and luminescence spectroscopy, two copper(I) are thereby quantitatively transferred from Cu(I)CopZ to Zn(II)CopY, with displacement of the zinc(II) and transfer of copper from a non-luminescent, exposed, binding site in CopZ to a luminescent, solvent shielded, binding site in CopY.
Author(s): Cobine P, Wickramasinghe WA, Harrison MD, Weber T, Solioz M, Dameron CT
Publication type: Article
Publication status: Published
Journal: FEBS Letters
Print publication date: 19/02/1999
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
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