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Lookup NU author(s): Dr Katherine Deane
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T cell-mediated immunity is important in the control of chlamydia infection but chlamydia-specific T cells are also implicated in the inflammation and tissue damage which characterize chlamydia associated diseases. To investigate target antigens of the T cell-mediated immune response to chlamydia infection, Chlamydia trachomatis-specific CD4+ T cell clones were isolated from a patient with chlamydia-induced reactive arthritis. T cell immunoblotting indicated that an antigen of approximately 60 kilodaltons molecular mass was recognized, and recombinant 60 kilodalton cysteine-rich outer membrane 2 (OMP2) proved to be stimulatory. By using deletion constructs and synthetic peptides an epitope presented by HLA-DRB1*0401 was defined and proved to contain the nonamer peptide within the OMP2 sequence predicted to have the greatest binding affinity for DRB1*0401 The sequence of the epitope is conserved in all C. trachomatis strains but not in C. pneumoniae. Investigation of patients with acute urethritis and additional patients with sexually acquired reactive arthritis showed that OMP2-reactive T cells were readily detectable in peripheral blood and synovial fluid. Thus OMP2 is a target antigen of the T cell-mediated immune response to CT infection.
Author(s): Deane KH; Goodall JC; Beacock-Sharp H; Gaston JS
Publication type: Article
Publication status: Published
Journal: Clinical & Experimental Immunology
Year: 2001
Volume: 126
Issue: 3
Pages: 488-493
ISSN (print): 0009-9104
ISSN (electronic): 1365-2249
Publisher: Wiley-Blackwell Publishing Ltd.
URL: http://dx.doi.org/10.1046/j.1365-2249.2001.01709.x
DOI: 10.1046/j.1365-2249.2001.01709.x
Notes: Goodall JC Beacock-Sharp H Deane KH Gaston JS England In Vitro Journal Article Research Support, Non-U.S. Gov't
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