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Identification of Theileria annulata in cattle using different molecular techniques: International comparison

Lookup NU author(s): Dr Olivier Sparagano


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Seven laboratories decided to compare their molecular techniques (either PCR or RLB) to identify Theileria annulata within 120 cattle blood samples. For clinical samples all the laboratories detected the pathogen. Among the 24 samples originating from an area where T. annulata has not been identified, five samples were observed positives from at least one laboratory. For the other 72 samples a unanimous results was observed for 50.7 p. cent samples. RLB gave higher positive rates than PCR and a lower discrepancy between laboratories performing it.

Publication metadata

Author(s): Sparagano O, Bouattour A, Caarelli G, Shkap V, Vitale F, Habela M, Almeria S, Castella J, Corchero E, Loria GR, Molad T, Ceci L

Publication type: Article

Publication status: Published

Journal: AEEMA Meeting: Epidémiologie et Santé Animale

Year: 2000

Volume: 38

Pages: 63-65

Publisher: E.N.V.A.