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Diversity among aromatic hydrocarbon-degrading bacteria and their meta-cleavagegenes

Lookup NU author(s): Professor Ian Head

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Abstract

Sixty-one strains of bacteria capable of growth on 4-methyl benzoic acid (29 isolates) or naphthalene (32 isolates) as the sole source of carbon and energy were isolated from sediments and water samples from the River Tyne, UK. Random amplification of polymorphic DNA from genomic DNA extracted from the different strains demonstrated that 14 of the 4-methyl benzoate-degrading isolates were unique and the remainder fell into seven groups containing two or three isolates that produced identical banding patterns. Thirteen of the naphthalene-degrading isolates were unique and nine groups with two or three identical representatives encompassed all other isolates. Screening of the bacterial strains for the presence of genes homologous to xylE, nahC and bphC by polymerase chain reaction and dot blot hybridization demonstrated that most strains harboured xylE- and/or nahC-like genes and only a single isolate was found that did not harbour any of these genes. None of the isolates harboured bphC-like genes. It was concluded that, while considerable diversity existed in host strains isolated using a single simple enrichment procedure, the extradiol dioxygenase genes involved in aromatic ring cleavage, present in these strains, were conserved to a considerable degree.

Publication metadata

Author(s): Daly K, Dixon AC, Swannell RPJ, Lepo JE, Head IM

Publication type: Article

Publication status: Published

Journal: Journal of Applied Microbiology

Year: 1997

Volume: 83

Issue: 4

Pages: 421-429

ISSN (print): 1364-5072

ISSN (electronic): 1365-2672

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1046/j.1365-2672.1997.00254.x

DOI: 10.1046/j.1365-2672.1997.00254.x

PubMed id: 9351224

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