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Androgen regulation of ornithine decarboxylase in human prostatic cells identified using differential display

Lookup NU author(s): Ian Waite, Professor David Neal, Professor Craig Robson


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Androgens are essential for normal prostate physiology and have a permissive role in the development and progression of prostate cancer. Using the mRNA differential display technique, ornithine decarboxylase (ODC) was identified to be up-regulated by androgens in human prostatic LNCaP cells. On Northern analysis, the induction of ODC expression by 10 nM androgen was rapid and continued up to 48 h exposure with a maximum 6.3-fold up-regulation. The anti-androgen Casodex inhibited the androgen-induced up-regulation of DC, whereas the protein synthesis inhibitor cycloheximide did not. Together these data suggest that regulation is mediated through the androgen receptor protein and does require secondary protein synthesis, respectively. The kinetics of induction of ODC were almost identical to those of prostate specific antigen. Taken together these data suggest that ODC is directly regulated by androgens in LNCaP cells.

Publication metadata

Author(s): Betts, A. M., Waite, I. S., Neal, D. E., Robson, C. N.

Publication type: Article

Publication status: Published

Journal: FEBS Letters

Year: 1997

Volume: 405

Issue: 3

Pages: 328-332

Print publication date: 01/04/1997

ISSN (print): 0014-5793

ISSN (electronic): 1873-3468


DOI: 10.1016/S0014-5793(97)00209-3

PubMed id: 9108313


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