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A putative DNA repair operon in B. subtilis comprising a gene encoding a DNA repair polymerase, a routs homolog and an unidentified

Lookup NU author(s): Professor Anil Wipat, Dr Zoltan Pragai, Jane Guy, Professor Colin Harwood


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As part of an international project to sequence the genome of Bcleillus ultilis. we have sequenced the 114 kb region from 240 to 256 degrees. Analysis of open reading frames revealed three genes yshC. yshD and yshE which appear to lie in an operon of L6 kb in length which may have a role in DNA repair. Analysis of the ysh gene product sequence showed that it possessed homology to I)NA polymerase beta from a number of organisms including Thermus aquaticus, Crithidia fasciculata, humans and rats. The putative product of the yshD gene was homologous to the products of routs from a variety of organisms including Synechocystis sp.. Thermus aquaticus. E. cell and 14. subtilis, and the equivalent genes MSH3 in mouse and hMSIi3 in humans, all of which are enzymes responsible for heteroduplex DNA base mismatch repair. This B. subtilis mute homolog is distinct from the previously identified routS gene in the routS" mutL operon located at approximately 150 degrees on the B. subtilis genome. The product of the final gene in the operon, yshE could not be identified by data base comparisons. In possessing at least two routs genes, B. subtilis is apparently similar to enkaryotes but different from gram negative bacteria such as E. coll. H. influerzae, and CoalmoTella which have only one rnutoc gene. We are presently studying the control and function of the three genes in the putative B.ubtilis repair operon by largeted muaeneis using the plasmid pMutin2, and by over xpression and purification proteins.

Publication metadata

Author(s): Fnlmerson P-I, Brignell C, Wipat A, Cartez N, Pragia Z, Guy BJ, Harwood CR

Publication type: Article

Publication status: Published

Journal: FASEB Journal

Year: 1997

Volume: 11

Issue: 9

Pages: A1368-

Print publication date: 01/01/1997

ISSN (print): 0892-6638

ISSN (electronic):

Publisher: Federation of American Societies for Experimental Biology