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Lookup NU author(s): Professor Colin Brooks
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Although the patterns of lymphokine (LK) secretion by CD4 and CD8 αβ cells have been extensively studied, the question of whether γδ T cells display patterns of restricted LK production and whether these patterns are the same as seen in conventional αβ T cells has not been previously addressed. In this study we generated panels of γδ T cell clones from normal C57BL/6 and BALB/c mice using a lectin-driven system and compared their patterns of secretion of nine LK with those of CD4 and CD8 αβ T cell clones generated in the same system. The results showed that γδ T cell clones displayed nonrandom patterns of highly restricted LK production with a strong bias towards the production of type 1 LK. The dominant pattern was one of high level secretion of interferon-γ and tumor necrosis factor (TNF), with variable production of interleukin (IL)-2, and little or none of the type 2 LK IL-4, IL-5, IL-6, and IL-10. This pattern differed significantly from that of CD4 Th1 clones in that γδ clones showed a striking deficiency in the production of IL-3 and granulocyte/macrophage colony-stimulating factor. A small subset of γδ clones displayed a novel pattern, in which the only LK produced in substantial quantity were TNF and variable amounts of IL-2. The bias of γδ T cells towards type 1 LK production was not an artefact associated with cloning because bulk populations of splenic γδ T cells behaved in the same way, even when activated in the presence of high concentrations of IL-4.
Author(s): Duhindan N, Farley AJ, Humphreys S, Parker C, Rossiter B, Brooks CG
Publication type: Article
Publication status: Published
Journal: European Journal of Immunology
Year: 1997
Volume: 27
Issue: 7
Pages: 1704-1712
Print publication date: 01/07/1997
Online publication date: 23/11/2005
Acceptance date: 10/04/1997
ISSN (print): 0014-2980
ISSN (electronic): 1521-4141
Publisher: Wiley - VCH Verlag GmbH & Co. KGaA
URL: http://dx.doi.org/10.1002/eji.1830270717
DOI: 10.1002/eji.1830270717
PubMed id: 9247581
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