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Analysis of bacteriohopanepolyols in sediment and bacterial extracts by high performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry

Lookup NU author(s): Dr Paul Farrimond


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We report the new application of negative ion atmospheric pressure chemical ionization (APCI) to the detection of composite bacteriohopanepolyols in bacterial and sedimentary (or other natural environmental) samples. The method employs the addition of dichloromethane to the high performance liquid chromatography eluent prior to APCI to stimulate formation of chlorine addition/association ions from the hopanoid alcohols. This approach provided the first direct analysis of composite bacterial hopanoids in a sediment, identifying bacteriohopanetrol and bacteriohopanetetrol cyclitol ether.

Publication metadata

Author(s): Fox PA, Carter J, Farrimond P

Publication type: Article

Publication status: Published

Journal: Rapid Communications in Mass Spectrometry

Year: 1998

Volume: 12

Issue: 10

Pages: 609-612

Print publication date: 04/12/1998

ISSN (print): 0951-4198

ISSN (electronic): 1097-0231

Publisher: John Wiley & Sons Ltd.


DOI: 10.1002/(SICI)1097-0231(19980529)12:10<609::AID-RCM210>3.0.CO;2-U


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