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Lookup NU author(s): Junghyun Yoon,
Professor Michael Goodfellow
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Two rRNA gene clusters were detected in the genome of Bifidobacterium bifidum KCTC 3202(T) using Southern blot analysis. To analyse the sequences of the 16S rRNA genes from rrnA and rrnB, 16S rDNAs were amplified by PCR using DNA fragments purified from gel slices containing each of the rRNA gene clusters. The amplified 16S rDNAs from rrnA and rrnB were cloned into vectors and three clones of each gene sequenced. The resultant sequences were confirmed by direct sequencing of the 16S rDNAs from rrnA and rrnB. Sequence differences were not found between rrnA and rrnB in 1488 bp of the 16S rRNA genes.
Author(s): Yoon J-H, Yim DK, Goodfellow M, Park Y-H
Publication type: Article
Publication status: Published
Journal: Antonie van Leeuwenhoek
ISSN (print): 0003-6072
ISSN (electronic): 1572-9699
Publisher: Springer Netherlands
PubMed id: 10510720
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