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Lookup NU author(s): Dr Gavin Clowry
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The membrane-associated protein gephyrin can form part of the glycine receptor complex at inhibitory synapses. This study provides evidence for gephyrin localisation in the developing axons of the rat brain and spinal cord, with particular reference to the corticospinal tract. Using a well-characterised monoclonal antibody (MAb 7a) gephyrin-like immunoreactivity was found expressed by growing axons, disappearing as these axons became myelinated. Immunoelectron microscopy localised the antigen to the interior of small, unmyelinated axons in the dorsal funiculus of young rats. Western blot analysis of cervical spinal cord from different post-natal ages only detected one immunoreactive band at ages when immunohistochemistry revealed gephyrin-like immunoreactivity in both the grey matter and corticospinal tract. Furthermore, the molecular weight of this band corresponded to that of gephyrin, suggesting it is present both at synapses and in axons. The known tubulin binding ability of the gephyrin may have a role in stabilisation of microtubules in growing axons.
Author(s): Fallah Z, Clowry GJ
Publication type: Article
Publication status: Published
Journal: Developmental Neuroscience
Print publication date: 01/01/1999
ISSN (print): 0378-5866
ISSN (electronic): 1421-9859
Publisher: S. Karger AG
PubMed id: 10077702
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