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The role of calcium homeostasis and flux during bacterial antigen processing in murine macrophages

Lookup NU author(s): Dr Alexei von Delwig, Professor John Robinson

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Abstract

We report that MHC class II (MHC-II)-restricted antigen processing of two CD4+ T cell epitopes from the surface M protein of Streptococcus pyogenes in murine macrophages is dependent on intact calcium homeostasis and flux. We have previously shown that the CD4+ T cell epitope 308-319 of the type 5 M protein is presented by newly synthesized MHC-II molecules via the classical pathway, while 17-31 is loaded on recycling MHC-II molecules via the recycling pathway. In this report we show that processing of viable bacteria for 308-319 presentation depended on the availability of intra- and extra cellular calcium, intact gadolinium-sensitive and/or T-type calcium channels, as well as on thapsigargin-sensitive homeostasis of intracellular calcium. In contrast, processing of 17-31 was independent of both intracellular calcium and gadolinium-sensitive calcium channels. The data suggest that alternative antigen processing pathways have different requirements for intracellular calcium homeostasis.

Publication metadata

Author(s): von Delvig A, Robinson JH

Publication type: Article

Publication status: Published

Journal: European Journal of Immunology

Year: 1999

Volume: 29

Issue: 8

Pages: 2414-2419

Print publication date: 01/01/1999

ISSN (print): 0014-2980

ISSN (electronic): 1521-4141

Publisher: Wiley - VCH Verlag GmbH & Co. KGaA

URL: http://dx.doi.org/10.1002/(SICI)1521-4141(199908)29:08<2414::AID-IMMU2414>3.0.CO;2-P

DOI: 10.1002/(SICI)1521-4141(199908)29:08<2414::AID-IMMU2414>3.0.CO;2-P

PubMed id: 10458754

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