Browse by author
Lookup NU author(s): Professor Keith Chater
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
whiI is one of several loci originally described as essential for sporulation in Streptomyces coelicolor A3(2). We have characterized whiI at the molecular level. It encodes an atypical member of the response regulator family of proteins, lacking at least two of the residues strongly conserved in the conventional phosphorylation pocket. It is not adjacent to a potential sensor kinase gene. Fifteen mutant alleles of whiI were sequenced, revealing, among others, six mutations affecting conserved amino acids, several frameshift mutations and one mutation in the promoter. The whiI promoter is specifically transcribed by the sporulation-specific σ(WhiG)-containing form of RNA polymerase. Transcription of whiI is temporally controlled, reaching a maximum level coincident with the formation of spores. Further transcriptional studies suggested that WhiI is involved directly or indirectly in repressing its own expression and that of another σ(WhiG)-dependent sporulation-specific regulatory gene, whiH.
Author(s): Ainsa JA, Parry HD, Chater KF
Publication type: Article
Publication status: Published
Journal: Molecular Microbiology
Year: 1999
Volume: 34
Issue: 3
Pages: 607-619
Print publication date: 01/11/1999
ISSN (print): 0950-382X
ISSN (electronic): 1365-2958
Publisher: Wiley-Blackwell Publishing Ltd.
URL: http://dx.doi.org/10.1046/j.1365-2958.1999.01630.x
DOI: 10.1046/j.1365-2958.1999.01630.x
PubMed id: 10564501
Altmetrics provided by Altmetric
