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Astrocytes rather than neurones mediate interleukin-1β dependent nitric oxide and superoxide radical release in primary hypothalamic rat cell cultures

Lookup NU author(s): Emeritus Professor Calum McNeilORCiD


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The cellular sources of nitric oxide in the hypothalamus are thought to be 'NOergic' neurones. Using free radical electrochemical sensors we investigated nitric oxide and superoxide radical release in primary hypothalamic cell cultures. We present evidence that under interleukin-1β (IL-1β) stimulation hypothalamic astrocytes rather than neurones release nitric oxide. Under L-arginine deprivation and IL-1β stimulation a concentration-dependent release of superoxide was also observed, which was inhibited in the presence of nitric oxide synthase inhibitor nitro-L-arginine-methyl-ester. These findings support the hypothesis that the balance between nitric oxide and superoxide may be of vital importance in hypothalamic pathophysiology. Copyright (C) 1999 Elsevier Science Ireland Ltd.

Publication metadata

Author(s): Tolias CM, McNeil CJ, Kazlauskaite J, Hillhouse EW

Publication type: Article

Publication status: Published

Journal: Neuroscience Letters

Year: 1999

Volume: 273

Issue: 1

Pages: 57-60

Print publication date: 24/09/1999

ISSN (print): 0304-3940

ISSN (electronic): 1872-7972

Publisher: Elsevier Ireland Ltd


DOI: 10.1016/S0304-3940(99)00630-8

PubMed id: 10505651


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