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Confocal laser scanning microscopy of calcium dynamics in living cells

Lookup NU author(s): Emeritus Professor Michael Whitaker

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Abstract

Confocal laser scanning microscopy (CLSM) is widely used to monitor intracellular calcium levels in living cells loaded with calcium-sensitive fluorophores. This review examines the basic advantages and limitations of CLSM in in vivo imaging analyses of calcium dynamics. The benefits of utilizing ratioed images and dextran-conjugated fluorophores are addressed, and practical aspects of handling confocal datasets are outlined. After considering some relatively new microscopical methods that can be used in conjunction with conventional CLSM, possible future applications of confocal techniques in analyses of intracellular calcium dynamics are discussed.

Publication metadata

Author(s): Stricker SA, Whitaker M

Publication type: Article

Publication status: Published

Journal: Microscopy Research and Technique

Year: 1999

Volume: 46

Issue: 6

Pages: 356-369

Print publication date: 15/09/1999

ISSN (print): 1059-910X

ISSN (electronic): 1097-0029

Publisher: John Wiley & Sons, Inc.

URL: http://dx.doi.org/10.1002/(SICI)1097-0029(19990915)46:6<356::AID-JEMT4>3.0.CO;2-6

DOI: 10.1002/(SICI)1097-0029(19990915)46:6<356::AID-JEMT4>3.0.CO;2-6

PubMed id: 10504213

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