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Comparison of cytotoxic T-lymphocyte responses to hepatitis C virus core protein in uninfected and infected individuals

Lookup NU author(s): Dr Margaret Jackson, Professor Debra Bevitt, Emeritus Professor Geoffrey Toms, Professor Margaret Bassendine


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Cytotoxic T lymphocytes have been implicated in the control of hepatitis C virus (HCV) infection. Recognition by cytotoxic T lymphocytes of epitopes within HCV core protein has been defined previously by in vitro stimulation with synthetic peptides. The aim of this study has been to examine cytotoxic T-lymphocyte responses generated against peptides produced naturally following intracellular processing of viral protein. Antigen-specific cytotoxic T-lymphocyte lines were generated from both HCV uninfected and infected individuals by culturing CD8+ T cells with autologous dendritic cells loaded intracytoplasmically with recombinant HCV core protein. Analysis of the epitopes recognized by core protein-specific cytotoxic T lymphocytes used synthetic peptides that were selected based on their predicted binding to HLA-A * 0201 molecules. Core protein-specific cytotoxic T lymphocytes derived from HCV uninfected and infected individuals were able to lyse autologous target cells pulsed with each of 5 predicted epitopes. Generation of HCV-specific cytotoxic T lymphocytes using dendritic cells as antigen presenting cells provides a method of comparing the potential repertoire of cytotoxic T-lymphocyte responses to the responses that occur in chronically infected individuals. No evidence of a qualitatively different response by patient cytotoxic T lymphocytes was apparent which might explain persistence of the virus.

Publication metadata

Author(s): Jackson M, Smith B, Bevitt DJ, Steward M, Toms GL, Bassendine MF, Diamond AG

Publication type: Article

Publication status: Published

Journal: Journal of Medical Virology

Year: 1999

Volume: 58

Issue: 3

Pages: 239-246

Print publication date: 28/05/1999

ISSN (print): 0146-6615

ISSN (electronic): 1096-9071

Publisher: John Wiley & Sons, Inc.


DOI: 10.1002/(SICI)1096-9071(199907)58:3<239::AID-JMV9>3.0.CO;2-V

PubMed id: 10447419


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