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Lookup NU author(s): Dr Vincent Monchois,
Emeritus Professor Roy Russell
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Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C- terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was isolated.
Author(s): Monchois V, Arguello-Morales M, Russell RRB
Publication type: Article
Publication status: Published
Journal: Journal of Bacteriology
Print publication date: 01/04/1999
ISSN (print): 0021-9193
PubMed id: 10094712