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Isolation of an active catalytic core of Streptococcus downei MFe28 GTF- I glucosyltransferase

Lookup NU author(s): Dr Vincent Monchois, Emeritus Professor Roy Russell

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Abstract

Truncated variants of GTF-I from Streptococcus downei MFe28 were purified by means of a histidine tag. Sequential deletions showed that the C- terminal domain was not directly involved in the catalytic process but was required for primer activation. A fully active catalytic core of only 100 kDa was isolated.

Publication metadata

Author(s): Monchois V, Arguello-Morales M, Russell RRB

Publication type: Article

Publication status: Published

Journal: Journal of Bacteriology

Year: 1999

Volume: 181

Issue: 7

Pages: 2290-2292

Print publication date: 01/04/1999

ISSN (print): 0021-9193

ISSN (electronic):

PubMed id: 10094712

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