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Lookup NU author(s): Professor Bernard Connolly
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The secondary structure of an RNA aptamer, which has a high affinity for the Escherichia coli MetJ repressor protein, has been mapped using ribonucleases and with diethyl pyrocarbonate. The RNA ligand is composed of a stem-loop with a highly structured internal loop. Interference modification showed that the bases within the internal loop, and those directly adjacent to it, are important in the binding of the RNA ligand to MetJ. Most of the terminal stem-loop could be removed with little effect on the binding. Ethylation interference suggests that none of the phosphate groups are absolutely essential for tight binding. The data suggest that the MetJ binding site on the aptamer is distinct from that of the natural DNA target, the 8-base pair Met box.
Author(s): McGregor A, Murray JB, Adams CJ, Stockley PG, Connolly BA
Publication type: Article
Publication status: Published
Journal: Journal of Biological Chemistry
Print publication date: 22/01/1999
ISSN (print): 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology, Inc.
PubMed id: 9890988
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