Toggle Main Menu Toggle Search

Open Access padlockePrints

Stathmin is involved in S100A4-mediated regulation of cell cycle progression

Lookup NU author(s): Dr Gajanan Sherbet


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


S100A4 is a cell proliferation- and cancer metastasis-related gene. Previous studies have shown that over-expression of S100A4 drives the cells into the S-phase of the cell cycle, with concomitant enhancement of p53 detection. This has led to the postulate that S100A4 could be controlling cell cycle progression by sequestering p53 and abrogating its G1-S checkpoint control. Cells induced by S100A4 to enter the S-phase do successfully negotiate the G2-M checkpoint control. Here we show that S100A4 is also involved in the regulation of control at this checkpoint. Stathmin is known to be associated, together with p53 in controlling G2-M transition. We present evidence that the expression of S100A4 and stathmin genes is up regulated in exponentially growing HeLa cells. They are down regulated in parallel when cell proliferation is inhibited by hyperthermia and 4-hydroxynonenal (4-HNE). We postulate that S100A4 might directly induce stathmin up regulation to enable cells to enter into mitosis. Since wild-type p53 is known to down regulate stathmin expression, we further postulate this might also involve S100A4-mediated sequestration of p53. The expression of heme oxygenase (HO-1), a stress-response protein, has been used to monitor effects of hyperthermia, 12-O-tetradecanoly phorbol 13-acetate (TPA) and 4-HNE. All these treatments induced HO-1 and also when cells growing in serum-deficiency were restored with full serum. HO-1 induction occurred irrespective of S100A4 expression status. HO-1 gene has responsive elements for many angiogenic agents and induces marked neovascularisation of tumours. We suggest therefore that S100A4 may not possess angiogenic properties.

Publication metadata

Author(s): Cajone F, Sherbet GV

Publication type: Article

Publication status: Published

Journal: Clinical and Experimental Metastasis

Year: 1999

Volume: 17

Issue: 10

Pages: 865-871

Print publication date: 01/10/1999

ISSN (print): 0262-0898

ISSN (electronic): 1573-7276

Publisher: Springer


DOI: 10.1023/A:1006778804532

PubMed id: 11089885


Altmetrics provided by Altmetric