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Dysregulation of PMN antigen expression in type 2 diabetes may reflect a generalized defect of exocytosis: Influence of hypertension and microalbuminuria

Lookup NU author(s): Dr Peter Senior, Emerita Professor Sally Marshall, Dr Trevor Thomas

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Abstract

Defective exocytosis could underlie clinical and metabolic abnormalities in Type 2 diabetes. Because many SNARE proteins appear to be common mediators of exocytosis, we examined phorbol myristate acetate-stimulated expression of CD11b and CD69 on polymorphonuclear leukocytes (PMN) from Type 2 diabetic subjects with hypertension and microalbuminuria (D-htma), hypertension only (D-ht) or uncomplicated (D-uc), and normal controls (NC) by flow cytometry. CD11b expression was rapid (half maximal by 7 min), initially on all PMN. CD69 expression took place subsequently but on PMN that did not express CD11b. The proportion of CD11b-positive PMN at 30 min was higher in all diabetic groups than in NC. Expression of CD11b was higher and CD69 lower in D-uc and D-htma but were similar in NC and D-ht. In Type 2 diabetes the transition from the CD11b-positive to CD69-positive state is impaired. The defect in the process of CD69 expression appeared most marked in diabetic subjects with hypertension and microalbuminuria.

Publication metadata

Author(s): Marshall SM; Senior PA; Thomas TH

Publication type: Article

Publication status: Published

Journal: Journal of Leukocyte Biology

Year: 1999

Volume: 65

Issue: 6

Pages: 800-807

Print publication date: 01/06/1999

ISSN (print): 0741-5400

ISSN (electronic): 1938-3673

Publisher: Federation of American Societies for Experimental Biology

URL: http://www.jleukbio.org/content/65/6/800.abstract

PubMed id: 10380902

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