Toggle Main Menu Toggle Search

Open Access padlockePrints

Dysregulation of PMN antigen expression in type 2 diabetes may reflect a generalized defect of exocytosis: Influence of hypertension and microalbuminuria

Lookup NU author(s): Dr Peter Senior, Emerita Professor Sally Marshall, Dr Trevor Thomas


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Defective exocytosis could underlie clinical and metabolic abnormalities in Type 2 diabetes. Because many SNARE proteins appear to be common mediators of exocytosis, we examined phorbol myristate acetate-stimulated expression of CD11b and CD69 on polymorphonuclear leukocytes (PMN) from Type 2 diabetic subjects with hypertension and microalbuminuria (D-htma), hypertension only (D-ht) or uncomplicated (D-uc), and normal controls (NC) by flow cytometry. CD11b expression was rapid (half maximal by 7 min), initially on all PMN. CD69 expression took place subsequently but on PMN that did not express CD11b. The proportion of CD11b-positive PMN at 30 min was higher in all diabetic groups than in NC. Expression of CD11b was higher and CD69 lower in D-uc and D-htma but were similar in NC and D-ht. In Type 2 diabetes the transition from the CD11b-positive to CD69-positive state is impaired. The defect in the process of CD69 expression appeared most marked in diabetic subjects with hypertension and microalbuminuria.

Publication metadata

Author(s): Marshall SM; Senior PA; Thomas TH

Publication type: Article

Publication status: Published

Journal: Journal of Leukocyte Biology

Year: 1999

Volume: 65

Issue: 6

Pages: 800-807

Print publication date: 01/06/1999

ISSN (print): 0741-5400

ISSN (electronic): 1938-3673

Publisher: Federation of American Societies for Experimental Biology


PubMed id: 10380902