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The use of 16S rDNA methods in soil microbial ecology

Lookup NU author(s): Dr Andrew Macrae


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New and exciting molecular methods, many using the 16S small sub-unit ribosomal nucleic acid molecule, are opening the microbial "black box" in soil. These studies have added much to our knowledge of microbial diversity in soils, and are beginning to advance our understanding of the relationship between this diversity and its function in soil processes. Over the next few years, the knowledge gained from molecular studies will, we hope, lead to improvements in sustainable land management and sustainable exploitation of soil genetic resources. As we enter the third millenium, it is appropriate to review the application of 16S rDNA methods to soil microbiology. This review examines 16S ribosomal DNA (rDNA) methods and their application to soil. It mentions their limits and suggests how they may be applied in the future.

Publication metadata

Author(s): Macrae A

Publication type: Review

Publication status: Published

Journal: Brazilian Journal of Microbiology

Year: 2000

Volume: 31

Issue: 2

Pages: 77-82

Print publication date: 01/04/2000

ISSN (print): 1517-8382

ISSN (electronic): 1678-4405


DOI: 10.1590/S1517-83822000000200002