ePrints

Use of combined microautoradiography and fluorescence in situ hybridization to determine carbon metabolism in mixed natural communities of uncultured bacteria from the genus Achromatium

Lookup NU author(s): Emeritus Professor Neil GrayORCiD, Richard Howarth, Professor Ian Head

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).

Publication metadata

Author(s): Gray ND, Howarth R, Pickup RW, Jones JG, Head IM

Publication type: Article

Publication status: Published

Journal: Applied and Environmental Microbiology

Year: 2000

Volume: 66

Issue: 10

Pages: 4518-4522

ISSN (print): 0099-2240

ISSN (electronic): 1098-5336

Publisher: American Society for Microbiology

URL: http://dx.doi.org/10.1128/AEM.66.10.4518-4522.2000

DOI: 10.1128/AEM.66.10.4518-4522.2000

PubMed id: 11010908

Altmetrics

Share