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Variation in epidermal housekeeping gene expression in different pathological states

Lookup NU author(s): Professor Jonathan Rees


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Using non-radioactive in situ hybridization we studied the expression of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and PolyA+ RNA in psoriasis and normal skin, and GAPDH in epidermis following application of a range of noxious stimuli, including ultraviolet radiation, Sellotape stripping and various irritants. In keeping with what might have been expected from previous results on cell culture of various non-epidermal cell types, expression of these putative control gene products is not constant. We suggest that the use of GAPDH, and possibly other control genes, may lead to error in interpreting experiments using Northern blotting or even RT-PCR of epidermal samples.

Publication metadata

Author(s): Wu Yih-Yiing, Rees JL

Publication type: Article

Publication status: Published

Journal: Acta Dermato-Venereologica

Year: 2000

Volume: 80

Issue: 1

Pages: 2-3

Print publication date: 01/01/2000

ISSN (print): 0001-5555

ISSN (electronic): 1651-2057

Publisher: Taylor & Francis


PubMed id: 10721821