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An improved method for the immunological detection of mineral bound protein using hydrofluoric acid and direct capture

Lookup NU author(s): Dr Oliver Craig, Dr Matthew Collins

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Abstract

Immunological detection of proteins adsorbed to mineral and ceramic surfaces has proved not only difficult but controversial. Unlike the immunological detection of proteins associated with carbonate or phosphate minerals (e.g. shells and bones) proteins adsorbed to siliceous minerals cannot readily be removed by dissolution of the mineral phase. We have previously examined alternative extraction methodologies which claim to bring the protein into solution, but found none of these to be effective. Here we report a novel strategy for immuno-detection of proteins adsorbed to siliceous minerals, the Digestion and Capture Immunoassay (DACIA). The method involves the use of cold, concentrated (4M) hydrofluoric acid (HF) with the simultaneous capture of liberated protein onto a solid phase. The combination of low temperatures and surface stabilisation enables us to detect epitopes from even partially degraded proteins. The method may have a wide application in forensic, archaeological, soil and earth sciences. Copyright (C) 2000 Elsevier Science B.V.

Publication metadata

Author(s): Craig OE, Collins MJ

Publication type: Article

Publication status: Published

Journal: Journal of Immunological Methods

Year: 2000

Volume: 236

Issue: 1-2

Pages: 89-97

Print publication date: 06/03/2000

ISSN (print): 0022-1759

ISSN (electronic): 1872-7905

Publisher: Elsevier Science B.V.

URL: http://dx.doi.org/10.1016/S0022-1759(99)00242-2

DOI: 10.1016/S0022-1759(99)00242-2

PubMed id: 10699582

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