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Embryonic development in vitro is compromised by the ICSI procedure

Lookup NU author(s): Professor Alison Murdoch, Professor Mary Herbert


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The implantation rates achieved with intracytoplasmic sperm injection (ICSI) are equivalent to those with conventional in-vitro fertilization (IVF) but information on embryonic development in vitro after ICSI is scant. In this paper we compare blastocyst formation after IVF and ICSI; we have also investigated the effect of the ICSI procedure with internal control of extrinsic (including paternal) factors. The first series comprised cases of IVF treatment (n = 101) for tubal infertility and ICSI (n = 96) for male infertility. The proportions of embryos developing to the blastocyst stage was significantly lower after ICSI (8.9%, P < 0.001) than after conventional IVF (23.5%). In order to investigate the effect of the ICSI procedure in isolation, blastocyst formation was analysed in a second series of eight cases, in which sibling oocytes were non-selectively subjected to ICSI (n = 78) or IVF (n = 67) with spermatozoa from the same semen sample. It was found that 20% of ICSI embryos and 50% of IVF embryos formed blastocysts (P < 0.01), demonstrating that the ICSI procedure contributes to a reduced capacity for blastocyst formation in vitro.

Publication metadata

Author(s): Griffiths TA, Murdoch AP, Herbert M

Publication type: Article

Publication status: Published

Journal: Human Reproduction

Year: 2000

Volume: 15

Issue: 7

Pages: 1592-1596

Print publication date: 01/01/2000

ISSN (print): 0268-1161

ISSN (electronic): 1460-2350

Publisher: Oxford University Press


DOI: 10.1093/humrep/15.7.1592

PubMed id: 10875872


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