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Detection of cytomegalovirus (CMV) antigens in kidney biopsies and transplant nephrectomies as a marker for renal graft dysfunction

Lookup NU author(s): Dr Helen Robertson, Dr Marie O'Donnell, Samia Ali, David Talbot


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Chronic rejection accounts for the greatest loss of renal allografts. HLA mismatching has been minimised by organ allocation and new immunosuppressive drugs have been employed, but the average cadaveric graft survival still does not exceed 12 years. Though the aetiology is multifactorial, one contributory factor for this condition is cytomegalovirus (CMV). Detection of CMV in kidney biopsies and sera can diagnose and monitor this inflammatory event and define its role in chronic nephropathy. Twenty five biopsies taken at the time of transplantation, 10 biopsies for graft dysfunction and tissue blocks from 20 explanted kidney grafts were collected and investigated for CMV antigens by immunohistochemistry. Tissue samples were snap frozen and cryostat sections were incubated with monoclonal antibodies for CMV antigens followed by immunoperoxidase staining. In 12 out of 20 transplant nephrectomies CMV antigens were found. Only two of these patients had clinical CMV disease. Time 0 biopsies from CMV seronegative donors (n = 11) and CMV seropositive donors (n = 14) were negative for CMV antigens. The prevalence of CMV antigens in grafts lost due to chronic rejection was 60%. These antigens were not found within the time 0 biopsies, but were detected in 30% of biopsies taken at the time of clinical graft dysfunction. CMV appears to contribute to chronic rejection even without clinical disease.

Publication metadata

Author(s): Gerstenkorn C, Robertson H, Mohamed MA, O'Donnell M, Ali S, Talbot D

Publication type: Article

Publication status: Published

Journal: Clinical Chemistry and Laboratory Medicine

Year: 2000

Volume: 38

Issue: 11

Pages: 1201-1203

ISSN (print): 1434-6621

ISSN (electronic): 1437-4331

Publisher: Walter de Gruyter GmbH & Co.


DOI: 10.1515/CCLM.2000.188

PubMed id: 11156360


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