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Cytochemical evidence that acetylcholine is a neurotransmitter of neurons that make excitatory and inhibitory outputs in the locust ocellar visual system

Lookup NU author(s): Dr Gerd Leitinger, Dr Peter Simmons

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Abstract

Three different cytochemical methods were used to detect acetylcholine in large, second-order neurons of locust ocelli (L-neurons). The first method used polyclonal antibodies raised against choline cleaved from acetylcholine and then conjugated with native protein, and this revealed strong staining for acetylcholine in axons whose number, size, and location indicated that they were of L-neurons. A corresponding staining pattern was found using the second method with a polyclonal antiserum against choline acetyltransferase (ChAT). The third method was the histochemical detection at the electron microscope level of acetylcholinesterase, the enzyme responsible for the breakdown of acetylcholine. We found that this enzyme is located in synaptic clefts of L-neurons in both of the brain regions where L-neurons are known to make excitatory and inhibitory output synapses. Acetylcholinesterase was confined to synaptic sites, which is consistent with a role in synaptic transmission at these synapses. Taken together, the findings suggest that L- neurons use acetylcholine as a neurotransmitter.


Publication metadata

Author(s): Leitinger G; Simmons PJ

Publication type: Article

Publication status: Published

Journal: Journal of Comparative Neurology

Year: 2000

Volume: 416

Issue: 3

Pages: 345-355

ISSN (print): 0021-9967

ISSN (electronic): 0092-7317

Publisher: John Wiley & Sons

URL: http://dx.doi.org/10.1002/(SICI)1096-9861(20000117)416:3<345::AID-CNE6>3.0.CO;2-T

DOI: 10.1002/(SICI)1096-9861(20000117)416:3<345::AID-CNE6>3.0.CO;2-T

PubMed id: 10602093


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