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Lookup NU author(s): Emerita Professor Anne Borland, Dr Tahar Taybi
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Isolation of high quality, high molecular weight RNA from plant tissues is complicated by high levels of polyphenols and polysaccharides which bind to and/or co-precipitate with RNA. Using high molecular weight polyethylene glycol (HMW-PEG), RNA was successfully isolated from plant species in which other RNA extraction methods and commercially available kits failed to deliver suitable results. We tested various buffer systems and isolation conditions with and without PEG or PVP (polyvinylpyrrolidone) using tissue from species of Aloe, Ananas, Clusia, Euphorbia, Kalanchoe, Opuntia, and Pyrrosia, all of which contain high amounts of phenolic compounds and/or polysaccharides. HMW-PEG was found to be more effective than PVP in removing these compounds. RNA extraction using HMW-PEG resulted in RNA of high quality from all species investigated, as indicated by UV light absorption profiles, and also yielded PCR amplification products after reverse transcription.
Author(s): Gehrig HH, Winter K, Cushman J, Borland A, Taybi T
Publication type: Article
Publication status: Published
Journal: Plant Molecular Biology Reporter
Year: 2000
Volume: 18
Issue: 4
Pages: 369-376
Print publication date: 01/01/2000
ISSN (print): 0735-9640
ISSN (electronic): 1572-9818
Publisher: Springer New York LLC
URL: http://dx.doi.org/10.1007/BF02825065
DOI: 10.1007/BF02825065
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