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Overexpression of the crgA gene abolishes light requirement for carotenoid biosynthesis in Mucor circinelloides

Lookup NU author(s): Dr Victor Ruiz-Perez

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Abstract

This work describes the isolation and characterization of crgA, a Mucor circinelloides gene, which has a dominant-positive effect on light-regulated carotenogenesis. The crgA gene was originally identified in a transformation experiment as a 3'-truncated open reading frame which caused carotenoid overaccumulation in the dark. The complete cloning and sequencing of crgA revealed that its putative product presented several recognizable structural domains: a RING-finger zinc binding domain near the N-terminus, a putative nuclear localization signal, two stretches of acidic amino acids, glutamine- rich regions and a putative isoprenylation motif. The expression of exogenous copies of the complete crgA gene or two different 3'-truncated versions, produced a similar dominant-positive effect on the light-inducible carotenogenesis of M. circinelloides. The presence of these exogenous sequences also caused a missregulation of the endogenous crgA gene, resulting in its overexpression. Collectively, these observations suggest that crgA is involved in the regulation of carotenoid biosynthesis by light.

Publication metadata

Author(s): Navarro E, Ruiz-Perez VL, Torres-Martinez S

Publication type: Article

Publication status: Published

Journal: European Journal of Biochemistry

Year: 2000

Volume: 267

Issue: 3

Pages: 800-807

ISSN (print): 0014-2956

ISSN (electronic): 1432-1033

Publisher: Wiley-Blackwell Publishing Ltd.

URL: http://dx.doi.org/10.1046/j.1432-1327.2000.01058.x

DOI: 10.1046/j.1432-1327.2000.01058.x

PubMed id: 10651817

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