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Lookup NU author(s): Dr Victor Ruiz-Perez
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This work describes the isolation and characterization of crgA, a Mucor circinelloides gene, which has a dominant-positive effect on light-regulated carotenogenesis. The crgA gene was originally identified in a transformation experiment as a 3'-truncated open reading frame which caused carotenoid overaccumulation in the dark. The complete cloning and sequencing of crgA revealed that its putative product presented several recognizable structural domains: a RING-finger zinc binding domain near the N-terminus, a putative nuclear localization signal, two stretches of acidic amino acids, glutamine- rich regions and a putative isoprenylation motif. The expression of exogenous copies of the complete crgA gene or two different 3'-truncated versions, produced a similar dominant-positive effect on the light-inducible carotenogenesis of M. circinelloides. The presence of these exogenous sequences also caused a missregulation of the endogenous crgA gene, resulting in its overexpression. Collectively, these observations suggest that crgA is involved in the regulation of carotenoid biosynthesis by light.
Author(s): Navarro E, Ruiz-Perez VL, Torres-Martinez S
Publication type: Article
Publication status: Published
Journal: European Journal of Biochemistry
ISSN (print): 0014-2956
ISSN (electronic): 1432-1033
Publisher: Wiley-Blackwell Publishing Ltd.
PubMed id: 10651817
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