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Lookup NU author(s): Dr Christine Masterson, Adrian Blackburn, Dr Cliff Wood
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Acyl-CoA dehydrogenase activity has been measured in homogenates of post-imbibition to 14 day-old hydroponically grown pea seeds at daily intervals, using C4, C12 and C16 acyl-CoA substrates. The activity peaks of the different chain-length acyl-CoA dehydrogenases did not transpose at all points and the ratios of the chain-length activities were not constant. It therefore has to be concluded that more than one dehydrogenase is present in pea mitochondria. There was a post-imbibition initial surge of activity with short- and mid-chain-length substrates. The C16-handling enzyme first peaked at 3-4 days, which coincided with the onset of plumule unfurling and greening. Further peaks were observed with all three substrates, coinciding with secondary root formation and leaf enlargement and later with cotyledon degeneration. Overall activity showed that the long-chain acyl-CoA dehydrogenase was much more active than the short-chain acyl-CoA dehydrogenase.
Author(s): Masterson C, Blackburn A, Wood C
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: 14th International Symposium on Plant Lipids
Year of Conference: 2000
Pages: 760-762
ISSN: 0300-5127
Publisher: Biochemical Society Transactions
URL: http://dx.doi.org/10.1042/0300-5127:0280760
DOI: 10.1042/0300-5127:0280760
PubMed id: 11171198