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The temporal and spatial distribution of p21(WAF) expression in skin appendages

Lookup NU author(s): Professor Jonathan Rees


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p21(WAF) is a cyclin-dependent kinase inhibitor which is widely expressed in epidermal structures. Using a combination of double immunocytochemical staining and combined in situ hybridization, we show that there is a striking exclusivity between the expression of Ki67 and p21(WAF) in the hair matrix. Some cells that are Ki67-positive also express p53, but as they exit the cell cycle they assume p21(WAF)-positive/p53-negative status. By contrast, cells in the interfollicular epidermis of psoriatic lesions, in the sebaceous gland, and in the outer root sheath are p21(WAF)- positive/p53-positive but Ki67-negative. These results suggest that in some anatomical parts of the epidermis, p21(WAF) expression can accompany p53 expression, whereas in other parts, the expression of these markers is reciprocal, suggesting that other pathways may be controlling p21(WAF) expression. In order to define, functionally, the presence of p53-independent p21(WAF) expression in skin, we examined lesions of Bowen's disease in which both alleles of p53 were inactivated. p21(WAF) expression was still observed, confirming a role for p53-independent expression of p21(WAF) in human skin in vivo.

Publication metadata

Author(s): Wu Y-Y, Takata M, Rehman I, Rees JL

Publication type: Article

Publication status: Published

Journal: British Journal of Dermatology

Year: 2000

Volume: 142

Issue: 4

Pages: 694-701

ISSN (print): 0007-0963

ISSN (electronic): 1365-2133

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1046/j.1365-2133.2000.03414.x

PubMed id: 10792219


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