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Lookup NU author(s): Dr Lai Siew, John Dessi, Julia SpoorsORCiD, Dr Larry Winger, Emeritus Professor Colin Self
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Although polyclonal antibodies specific for the herbicide imazapyr have been described and have formed the basis of an immunoassay (at one time commercially available but since withdrawn), it is clear that this molecule has limited immunogenicity. We evaluated various hapten-protein conjugates for their capacity to elicit antibody binding to imazapyr from multiply immunized murine hosts, as assessed by hapten-competed inhibition of reactivity with hapten conjugated to a heterologous carrier. From one highly reactive individual, we subsequently developed a monoclonal antibody exhibiting a high reactivity to this agrochemical. With this antibody we have been able to configure immunoassays in formats suitable for either laboratory or field use that exhibit both high performance and convenience, such that sensitivities for imazapyr in the range of 1 ng/ml can be achieved. While further testing of this anti-imazapyr antibody in field conditions is required, the availability of a constant source of immunoreactive immunoglobulin may be important in the development of useful immunoanalytical approaches for the assessment of imazapyr at the point-of-need. © 2002 Wiley Periodicals, Inc.
Author(s): Winger L; Dessi JL; Self CH; Spoors JA; Siew LK; Jennens L
Publication type: Article
Publication status: Published
Journal: Field Analytical Chemistry and Technology
Year: 2001
Volume: 5
Issue: 6
Pages: 281-287
Print publication date: 01/01/2001
ISSN (print): 1086-900X
ISSN (electronic): 1520-6521
Publisher: John Wiley & Sons
URL: http://dx.doi.org/10.1002/fact.10012
DOI: 10.1002/fact.10012
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