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Lookup NU author(s): Dr Margaret Dunn, Emeritus Professor Monica Hughes
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Two winter barley (Hordeum vulgare L. cv. Igri) genomic clones, λgblt101.1 and λgblt101.2, encoding the blt101 gene family, were isolated from a genomic library. Deletion analysis of the blt101.1 promoter, using transient β-glucuronidase (GUS) reporter expression assays, indicated that it contains at least three regulatory regions. A 107-bp region between nucleotides-168 and -275 with respect to the translation initiation codon, confers high -level GUS reporter expression at low temperature and contains a sequence (designated CR1) that is highly conserved in equivalent positions within the promoters of both members of the blt101 gene family. A 10-bp motif contained within CRt binds proteins present in nuclear extracts from both control and low-temperature-treated barley tissue. Loss-of-function experiments, using transient-expression analysis, confirmed that this motif acts as a previously unreported low-temperature-responsive element. Nuclease sensitivity analysis of intact chromatin indicated that the blt101.1 promoter becomes more susceptible to DNase and micrococcat nuclease at low temperature, consistent with chromatin reorganisation upon transcriptional induction. It is proposed that both the 10-bp motif and chromatin reorganisation are involved in the regulation of blt101.1 at low temperature. This is the first detailed analysis of a low-temperature-specific plant promoter and identifies a novel low-temperature-response element.
Author(s): Brown APC, Dunn MA, Goddard NJ, Hughes MA
Publication type: Article
Publication status: Published
Journal: Planta
Year: 2001
Volume: 213
Issue: 5
Pages: 770-780
Print publication date: 01/01/2001
ISSN (print): 0032-0935
ISSN (electronic): 1432-2048
Publisher: Springer
URL: http://dx.doi.org/10.1007/s004250100549
DOI: 10.1007/s004250100549
PubMed id: 11678282
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