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A gene trap integration provides an early in situ marker for hepatic specification of the foregut endoderm

Lookup NU author(s): Dr Elizabeth Jones, Dr David Wilson

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Abstract

We report the characterization of a gene trap integration that provides an in situ marker for one of the earliest events in liver development. Expression of the reporter gene is observed at the nine-somite stage in the hepatic field of the foregut endoderm. At 10.5 days post-coitus expression is observed exclusively and at high levels in the majority of cells in the developing liver bud. As development proceeds the proportion of expressing cells decreases with expression in adult liver being restricted to a few sporadic cells. This therefore provides the earliest, most specific in situ marker of the hepatic lineage reported to date and will be useful in the further characterization of the inductive events involved in hepatic specification. Molecular characterization of the gene trap insertion suggests that the expression pattern is the result of alternative promoter use in the ankyrin repeat-containing gene, gtar. Copyright © 2001 Elsevier Science Ireland Ltd.


Publication metadata

Author(s): Jones EA; Wilson DI; Watt AJ; Ure JM; Peddie D; Forrester LM

Publication type: Article

Publication status: Published

Journal: Mechanisms of Development

Year: 2001

Volume: 100

Issue: 2

Pages: 205-215

ISSN (print): 0925-4773

ISSN (electronic): 1872-6356

Publisher: Elsevier Ireland Ltd

URL: http://dx.doi.org/10.1016/S0925-4773(00)00530-X

DOI: 10.1016/S0925-4773(00)00530-X

PubMed id: 11165478


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