ePrints

Effect of aldosterone on collagen steady state levels in primary and subcultured rat hepatic stellate cells

Lookup NU author(s): Professor Albert Geerts

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

Background/Aims: Activation of the renin-angiotensin-aldosterone system can lead to collagen accumulation and reactive myocardial fibrosis. This study aims at evaluating the effect of aldosterone on extracellular matrix synthesis by rat hepatic stellate cells. Methods: Cultured cells were treated with different concentrations of aldosterone (10-6-10-10 M) and metabolically labeled with 35S-methionine/35S-cysteine. Procollagen types I, III and IV, laminin and fibronectin were specifically immunoprecipitated and quantified by phosphor imaging. Using the reverse transcription-polymerase chain reaction, we investigated the expression of the mineralocorticoid receptor in hepatic stellate cells. Results: Quantitation showed that 10-6 M aldosterone induced procollagen type I synthesis significantly, whereas procollagen type IV expression was significantly affected by 10-9 and 10-10 M aldosterone, both in primary hepatic stellate cells. RT-PCR experiments clearly demonstrated a lack of expression of the mineralocorticoid receptor in hepatic stellate cells. Conclusion: We demonstrated that aldosterone altered moderately procollagen type I and IV synthesis by primary hepatic stellate cells, but not by activated stellate cells which are the principal cellular sources of extracellular matrix proteins in chronic liver disease. Moreover, hepatic stellate cells do not express the mineralocorticoid receptor, suggesting that the observed modest changes of extracellular matrix synthesis are probably due to mineralocorticoid receptor unrelated mechanisms. © 2001 European Association for the Study of the Liver.

Publication metadata

Author(s): Rombouts K, Niki T, Wielant A, Hellemans K, Schuppan D, Kormoss N, Geerts A

Publication type: Article

Publication status: Published

Journal: Journal of Hepatology

Year: 2001

Volume: 34

Issue: 2

Pages: 230-238

ISSN (print): 0168-8278

ISSN (electronic): 1600-0641

Publisher: Elsevier B.V.

URL: http://dx.doi.org/10.1016/S0168-8278(00)00087-8

DOI: 10.1016/S0168-8278(00)00087-8

PubMed id: 11281551

Altmetrics

Share