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Oxidative stress signalling in the apoptosis of Jurkat T-lymphocytes

Lookup NU author(s): Dr Gajanan Sherbet

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Abstract

The pathways of transduction of oxidative stress signals have been studied using the Jurkat T cell model. The oxidative stress was induced by exposure of the cells to 100 μM H2O2. DNA damage was detected within 15 min after commencement of treatment. DNA damage repair occurred within about 1 h in cells exposed to oxidative stress for 15 min. In continuous exposure to stress, DNA repair was slower and control levels of DNA integrity were not reached. DNA repair did not involve gene transcription. H2O2 at 100 μM caused cell death by necrosis as well as by apoptosis. Both these processes were induced by 15 min exposure to the stress stimulus. However, some important differences were found between necrosis and apoptosis. Necrosis was more rapid, began within an hour of treatment and continued to increase during the full duration of the experiment. But apoptosis was seen after 4 h from treatment and was conspicuous between 6 and 20 h after the start of treatment. The necrotic phase preceded apoptosis, although these did show an overlap. In the necrotic phase, Bcl-2, Caspase 8 genes were down regulated. The 6-20 h phase characterised by a marked increase in apoptosis is accompanied by the up regulation of both Bcl-2 and Caspase genes. Expression of the Fas and p53 genes was not altered in either phase. We also analysed the levels of expression of the scavenging genes whose gene products are involved in detoxification. No modulation of the antioxidant enzymes, catalase, Cu/Zn superoxide dismutase and glutatione peroxidase was detectable. © 2001 Wiley-Liss, Inc.


Publication metadata

Author(s): Chiaramonte R, Bartolini E, Riso P, Calzavara E, Erba D, Testolin G, Comi P, Sherbet GV

Publication type: Article

Publication status: Published

Journal: Journal of Cellular Biochemistry

Year: 2001

Volume: 82

Issue: 3

Pages: 437-444

ISSN (print): 0730-2312

ISSN (electronic): 1097-4644

Publisher: John Wiley & Sons

URL: http://dx.doi.org/10.1002/jcb.1158

DOI: 10.1002/jcb.1158

PubMed id: 11500920


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