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Lookup NU author(s): Dr Olivier Sparagano
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A reverse line blot hybridisation (RLB) of 21 oligonucleotides with polymerase chain reaction (PCR) amplified regions of 16S rRNA (Ehrlichia/Anaplasma group) or 18S rRNA (Babesia/Theileria group) genes of haemoparasites detected Theileria annulata, T. buffeli/orientalis, Babesia bovis, B. bigemina, B. divergens, Ehrlichia bovis, Anaplasma marginale, A. centrale and unknown species within the Rickettsia tribe. A very high prevalence of mixed infections was detected, which indicated that animals infected with Babesia spp. were also infected with Theileria spp. and/or Anaplasma spp. The tick distribution appeared to be seasonal with Hyalomma marginatum as the most frequently observed tick and Boophilus annulatus and Ixodes ricinus as the least frequently observed ticks. Other species identified in the 818 ticks collected during the five sampling periods between April 1998 and November 1999 included H. lusitanicum, Rhipicephalus sanguineus group, R. bursa, Dermacentor marginatus, Haemaphysalis punctata, B. annulatus and I. ricinus. © 2001 Elsevier Science B.V. All rights reserved.
Author(s): Sparagano O; Georges K; Loria GR; Riili S; Greco A; Caracappa S; Jongejan F
Publication type: Article
Publication status: Published
Journal: Veterinary Parasitology
ISSN (print): 0304-4017
ISSN (electronic): 1873-2550
Publisher: Elsevier BV
PubMed id: 11511414
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