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Lookup NU author(s): Dr Mohamed Hamid,
Professor Michael Goodfellow
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16S ribosomal DNA (rDNA) and 16S-23S internal transcribed spacer rDNA sequence analyses were performed on Mycobacterium farcinogenes and M. senegalense strains and 26 strains of other rapidly growing mycobacteria to investigate the phylogenetic structure of bovine farcy mycobacteria within the M. fortuitum complex. M. farcinogenes and M. senegalense were indistinguishable in their 5′-end 16S rDNA but showed both considerable interspecies spacer sequence divergence and a high level of intraspecies sequence stability. A rapid detection assay using PCR and hybridization with species specific probes was developed. The assay was specific among 46 species other than M. farcinogenes and M. senegalense and correctly identified all M. farcinogenes and M. senegalense strains. PCR- and 16S-23S rDNA sequence-based detection will be a valuable approach for diagnosis of the causal agents of African bovine farcy in cattle.
Author(s): Goodfellow M; Hamid ME; Roth A; Landt O; Kroppenstedt RM; Mauch H
Publication type: Article
Publication status: Published
Journal: Journal of Clinical Microbiology
ISSN (print): 0095-1137
ISSN (electronic): 1098-660X
Publisher: American Society for Microbiology
PubMed id: 11826003
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