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NK cells developing in vitro from fetal mouse progenitors express at least one member of the Ly49 family that is acquired in a time-dependent and stochastic manner independently of CD94 and NKG2

Lookup NU author(s): Karen Fraser, Frances Davison, Professor John Robinson, Professor Colin Brooks


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NK cells developing in vitro from fetal progenitors in the presence of IL-2 are phenotypically and functionally indistinguishable from mature adult NK cells, with the exception that they generally lack surface expression of any of the Ly49 molecules that have previously been examined. Using two recently developed anti-Ly49 mAb, we show here that most of these NK cells in fact express high levels of at least one previously uncharacterized member of the Ly49 family, most likely Ly49E. Detailed kinetic and clonal analysis revealed that these Ly49 molecules were acquired in a progressive and stochastic manner independently of CD94 and NKG2. CD94 and NKG2 were both expressed early in NK cell development, sometimes in the absence of NK1.1, with CD94 invariably being expressed at two different levels. IL-4 differentially inhibited the expression of CD94 and Ly49 receptors, but had little or no effect on the expression of NKRP1 molecules.

Publication metadata

Author(s): Fraser KP, Gays F, Robinson JH, van Beneden K, Leclercq G, Vance RE, Raulet DH, Brooks CG

Publication type: Article

Publication status: Published

Journal: European Journal of Immunology

Year: 2002

Volume: 32

Issue: 3

Pages: 868-878

Print publication date: 01/03/2002

Online publication date: 28/02/2002

Acceptance date: 27/12/2001

ISSN (print): 0014-2980

ISSN (electronic): 1521-4141

Publisher: Wiley - VCH Verlag GmbH & Co. KGaA


DOI: 10.1002/1521-4141(200203)32:3<868::AID-IMMU868>3.0.CO;2-A

PubMed id: 11870631


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