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Lookup NU author(s): Professor Colin Harwood
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Aims: In Gram-positive bacteria, signal peptide-bearing secretory proteins are translocated through the cytoplasmic membrane and fold into their native conformation on the outside of the cell. The products of the Bacillus subtilis wprA and dltB genes separately influence post-translocational stages of the secretion process by mediating proteolytic degradation and folding of secretory proteins. Inactivation of either wprA or dltB in B. subtilis increases the yield of secretory proteins released into the culture medium in an intact and biologically active conformation. The aim of this work was to study the combined influence of these genes. Methods and Results: A wprA/dltB double mutant was constructed, but did not have an additive effect on secretion and caused a significant reduction in the yield of α-amylase. Conclusions and Significance: The activities of the wprA gene and the dlt operon interact in a negative way to influence the growth cycle and protein secretion. The mechanism by which this may occur, and its potential significance for the secretion of native and non-native proteins from B. subtilis and related bacteria, is discussed.
Author(s): Harwood CR; Stephenson K; Jensen CL; Jorgensen ST
Publication type: Article
Publication status: Published
Journal: Letters in Applied Microbiology
ISSN (print): 0266-8254
ISSN (electronic): 1472-765X
PubMed id: 12028417
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