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Comparative assessment expression of the inhibitor of growth 1 gene (ING1) in normal and neoplastic tissues

Lookup NU author(s): Dr Brian Angus, Professor John LunecORCiD, Dr Stephen Crosier, Andrew Lodge, Dr John Anderson


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Studies have indicated that the tumor suppressor p33ING1b (13q33-34) interact with p53. Moreover, the association of functional protein forms of each member of the p33ING1b/p53 complex is essential for optimum activity of p53. The present report describes the sequencing of cDNAs corresponding to the p33ING1b mRNAs in a series of normal and tumor cell lines, and the production of monoclonal antibodies (MAbs) reactive with p33ING1b. These antibodies were subsequently used to analyze p33ING1b expression in normal and tumor cell lines and tissues. No evidence of mutation of p33ING1b was found in any of the 15 tumor cell lines cDNAs studied. Our investigation of a wide range of normal tissues have shown that expression of the nuclear epitope is highly ubiquitous, whereas expression of the cytoplasmic form could be detected in only 50% of tissues studied. Considering neoplastic tissues, loss of nuclear p33ING1b was observed in melanoma, seminoma, papillary thyroid carcinoma, ductal breast carcinoma, and acute lymphoblastic leukemia. As with normal tissue, cytoplasmic p33ING1b was more restricted, being observed in around 30% of neoplastic tissues, but in melanoma, papillary thyroid carcinoma, ductal breast carcinoma, there was increased detection of cytoplasmic p33ING1b associated with concomitant loss of nuclear expression. These results may suggest that at least in some tumors, loss of effective p33ING1b function may be achieved by translocation to the cytoplasm or failure of nuclear localization.

Publication metadata

Author(s): Nouman, G., Angus, B., Lunec, J., Crosier, S, Lodge, A.J., Anderson, J.J.

Publication type: Article

Publication status: Published

Journal: Hybridoma and Hybridomics

Year: 2002

Volume: 21

Issue: 1

Pages: 1-10

Print publication date: 01/01/2002

ISSN (print): 1536-8599

ISSN (electronic): 1557-8348


DOI: 10.1089/15368590252917584

PubMed id: 11991811


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