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PCR and molecular detection for differentiating Vibrio species

Lookup NU author(s): Dr Olivier Sparagano

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Abstract

Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.

Publication metadata

Author(s): Sparagano OAE, Robertson PAW, Purdom I, McInnes J, Li Y, Yu D-H, Du Z-J, Xu H-S, Austin B

Publication type: Article

Publication status: Published

Journal: Annals of the New York Academy of Sciences

Year: 2002

Volume: 969

Issue: 1

Pages: 60-65

ISSN (print): 0077-8923

ISSN (electronic): 1749-6632

Publisher: Wiley-Blackwell Publishing, Inc.

URL: http://dx.doi.org/10.1111/j.1749-6632.2002.tb04351.x

DOI: 10.1111/j.1749-6632.2002.tb04351.x

PubMed id: 12381564

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