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Lookup NU author(s): Dr Olivier Sparagano
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Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.
Author(s): Sparagano OAE, Robertson PAW, Purdom I, McInnes J, Li Y, Yu D-H, Du Z-J, Xu H-S, Austin B
Publication type: Article
Publication status: Published
Journal: Annals of the New York Academy of Sciences
ISSN (print): 0077-8923
ISSN (electronic): 1749-6632
Publisher: Wiley-Blackwell Publishing, Inc.
PubMed id: 12381564
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