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Lookup NU author(s): Dr Gajanan Sherbet
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Two cytosine-adenine (CA) repeats CAR/CAL and RepIN20 occur in the human SEL1L gene, which is regarded as a candidate gene for insulin-dependent diabetes mellitus (IDDM) and Grave's disease. We have characterized these repeats to determine if they might serve as effective microsatellite markers for linkage analysis to clarify whether SEL1L gene plays a role in the pathogenesis of these autoimmune diseases. The allele frequencies and average heterozygosity of the microsatellite repeats were analysed in 94 DNA samples from peripheral blood mononuclear (PBMC) cells from adults of Northern Italy. The average heterozygosity was 0.68 for CAR/CAL polymorphism and 0.85 for RepIN20. The size of PCR fragments of CAR/CAL ranged from 207-225 bp and the most frequent allele was 207 bp (40.4%). The size of the fragments of RepIN20 ranged from 237-255 bp and the most frequent allele 249 bp (30.8%). In the light of the highly polymorphic nature of both microsatellites and their intragenic location in SEL1L gene, we suggest that they could provide a means for linkage analysis to clarify the potential role of SEL1L in conferring susceptibility to IDDM or Grave's disease.
Author(s): Chiaramonte R, Sabbadini M, Balordi F, Comi P, Sherbet GV
Publication type: Article
Publication status: Published
Journal: Molecular and Cellular Biochemistry
Year: 2002
Volume: 232
Issue: 1-2
Pages: 159-161
Print publication date: 01/01/2002
ISSN (print): 0300-8177
ISSN (electronic): 1573-4919
Publisher: Springer
URL: http://dx.doi.org/10.1023/A:1014828829621
DOI: 10.1023/A:1014828829621
PubMed id: 12030374
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