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Effect of starvation on proteases in insecticide-resistant and susceptible strains of Tribolium castaneum

Lookup NU author(s): Professor Mushtaq Saleem, Dr Richard WilkinsORCiD, Dr David Mantle


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To elucidate the possible involvement of the process of intracellular protein catabolism in the development of insecticide resistance, we have compared the activities of a comprehensive range of cytoplasmic and lysosomal proteolytic enzymes in 48 hours starved and un-starved resistant (CTC-12) and susceptible (FSS-11) strains of red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera: Tenebrionidae) under laboratory conditions. Compared to un-starved, the homogenates of 48 hours starved adult beetles of both resistant and susceptible strains showed elevated levels of all proteases tested in this study. In starved resistant beetles, all proteases except two were considerably increased which included alanyl aminopeptidase (41%), arginyl aminopeptidase (24%), leucyl aminopeptidase (154%), dipeptidyl aminopeptidase IV (27%), tripeptidyl aminopeptidase (26%), proline endopeptidase (316%), dipeptidyl aminopeptidase I (153%), dipeptidyl aminopeptidase II (50%), cathepsin B (22%) and cathepsin L (48%). On the other hand, in starved susceptible beetles, only two proteases viz lcucyl aminopeptidase (34%) and proline endopeptidase (66%) were increased, while the remaing showed less than 20% elevation. The results suggested that more number and higher induction of proteases in starved resistant than susceptible strain beetles may be important in the development of insecticide-resistance in T. castaneum.

Publication metadata

Author(s): Saleem MA, Wilkins RM, Mantle D, Shakoori AR

Publication type: Article

Publication status: Published

Journal: Pakistan Journal of Zoology

Year: 2003

Volume: 35

Issue: 3

Pages: 197-204

Print publication date: 01/01/2003

ISSN (print): 0030-9923

ISSN (electronic):

Publisher: Zoological Society of Pakistan