Browse by author
Lookup NU author(s): Dr Helen Talbot,
Dr Paul Farrimond
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Bacteriohopanepolyols (BHPs) fragment via characteristic pathways during atmospheric pressure chemical ionisation liquid chromatography/ion trap mass spectrometry (APCI-LC/MSn). Comparison of the MS2 spectra of bacteriohopane-32,33,34,35-tetrol (BHT) and 2β -methylbacteriohopane-32,33,34,35-tetrol has confirmed the previously proposed ring-C cleavage occurring between C-9 and 11 and C-8 and 14. This fragmentation, diagnostic of all hopanoids, also occurs in BHPs containing an amino group (-NH2) at C-35 although the higher relative stability of the ion limits this fragmentation to a minor process after protonation of the basic nitrogen function. Studies of a number of cell cultures including a prochlorophyte (Prochlorothrix hollandica) and a cyanobacterium (Chlorogloeopsis LA) demonstrate the power of this technique to detect composite BHPs with a complex biological functionality at C-35. We also report the first observation of intact pentafunctionalised bacteriohopanepolyols using this method. Copyright © 2003 John Wiley & Sons, Ltd.
Author(s): Talbot HM, Summons R, Jahnke L, Farrimond P
Publication type: Article
Publication status: Published
Journal: Rapid Communications in Mass Spectrometry
ISSN (print): 0951-4198
ISSN (electronic): 1097-0231
Publisher: John Wiley & Sons Ltd.
PubMed id: 14673828
Altmetrics provided by Altmetric