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Lookup NU author(s): Professor Miodrag Stojkovic
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During differentiation, somatic nuclei acquire highly specialized DNA and chromatin modifications, which are thought to result in cellular memory of the differentiated state [1]. Upon somatic nuclear transfer into oocytes, the donor nucleus may have to undergo reprogramming of these epigenetic marks in order to achieve totipotency. This may involve changes in epigenetic features similar to those that occur in normal embryos during early development [2-6]. However, there is accumulating evidence that epigenetic reprogramming is severely deficient in cloned embryos [7-12]. Several reports reveal inefficient demethylation and inappropriate reestablishment of DNA methylation in quantitative and qualitative patterns on somatic nuclear transfer [7-12]. Here we examine histone H3 lysine 9 (H3-K9) methylation and acetylation in normal embryos and in those created by somatic nuclear transfer. We find that H3-K9 methylation is reprogrammed in parallel with DNA methylation in normal embryos. However, the majority of cloned embryos exhibit H3-K9 hypermethylation associated with DNA hypermethylation, suggesting a genome-wide failure of reprogramming. Strikingly, the precise epigenotype in cloned embryos depends on the donor cell type, and the proportion of embryos with normal epigenotypes correlates closely with the proportion developing to the blastocyst stage. These results suggest a mechanistic link between DNA and histone methylation in the mammalian embryo and reveal an association between epigenetic marks and developmental potential of cloned embryos.
Author(s): Santos F, Zakhartchenko V, Stojkovic M, Peters A, Jenuwein T, Wolf E, Reik W, Dean W
Publication type: Article
Publication status: Published
Journal: Current Biology
Year: 2003
Volume: 13
Issue: 13
Pages: 1116-1121
ISSN (print): 0960-9822
ISSN (electronic): 1879-0445
Publisher: Cell Press
URL: http://dx.doi.org/10.1016/S0960-9822(03)00419-6
DOI: 10.1016/S0960-9822(03)00419-6
PubMed id: 12842010
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