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tr-kit promotes the formation of a multimolecular complex composed by Fyn, PLCγ1 and Sam68

Lookup NU author(s): Dr Julian Venables, Professor David Elliott


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tr-kit is a truncated form of the tyrosine kinase receptor c-kit expressed in the haploid phase of spermatogenesis. Upon microinjection, tr-kit triggers metaphase-to-anaphase transition in mouse eggs by the sequential activation of Fyn and PLCγ1. Here, we show that tr-kit promotes the interaction of several tyrosine-phosphorylated proteins with the SH3 domain of PLCγ1. Western blot analysis indicates that one of these proteins is Sam68, an RNA-binding protein that is known to interact with and be phosphorylated by Src-like kinases in mitosis. tr-kit promotes the association of Sam68 with PLCγ1 and Fyn in a multimolecular complex, as demonstrated by co-immunoprecipitation of the phosphorylated forms of these proteins using antibodies directed to anyone of the partners of the complex. Expression of tr-kit potentiates the interaction of endogenous Sam68 also with the SH3 domain of Fyn. Furthermore, the subcellular localization of Sam68 is affected by tr-kit through activation of Fyn in live cells. Lastly, we show that interaction with the SH3 domain of Fyn triggers the release of Sam68 from bound RNA. Thus, our data suggest that tr-kit promotes the formation of a multimolecular complex composed of Fyn, PLCγ1 and Sam68, which allows phosphorylation of PLCγ1 by Fyn, and may modulate RNA metabolism.

Publication metadata

Author(s): Elliott DJ; Venables JP; Paronetto MP; Geremia R; Rossi P; Sette C

Publication type: Article

Publication status: Published

Journal: Oncogene

Year: 2003

Volume: 22

Issue: 54

Pages: 8707-8715

ISSN (print): 0950-9232

ISSN (electronic): 1476-5594

Publisher: Nature Publishing Group

PubMed id: 14647465