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Lookup NU author(s): Romaan Jan Maria Raemaekers,
Professor Angharad MR Gatehouse
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The gene coding for agglutinin from Galanthus nivalis (GNA) was expressed in, and secreted by, the methylotrophic yeast, Pichia pastoris. Transformants of P. pastoris were selected and a process to produce and purify gram quantities of recombinant GNA was developed. GNA was secreted at approximately 80 mg l-1 at the 200 1 scale and was purified to 95% homogeneity using hydrophobic interaction chromatography. The recombinant protein was similar to the protein synthesised in plant with respect to structure and biological activity.
Author(s): Baumgartner P, Harper K, Raemaekers RJM, Durieux A, Gatehouse AMR, Davies HV, Taylor MA
Publication type: Article
Publication status: Published
Journal: Biotechnology Letters
Print publication date: 01/08/2003
ISSN (print): 0141-5492
ISSN (electronic): 1573-6776
PubMed id: 14514082
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