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Protein A-tagging for purification of native macromolecular complexes from Candida albicans

Lookup NU author(s): Christopher Blackwell, Dr Jeremy Brown


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Protein A-tagging has become an important tool in characterization of protein-protein interactions in many systems, allowing purification of multicomponent complexes under native conditions. Here we provide a set of vectors that allow protein A-tagging in Candida albicans, through addition of the tag to open reading frames. These vectors were successfully used to generate stably tagged proteins that were functional, shown to be localized appropriately or assembled into complexes. These new vectors comprise a useful addition to the C. albicans molecular toolbox. Copyright © 2003 John Wiley & Sons, Ltd.

Publication metadata

Author(s): Blackwell C, Russell CL, Argimon S, Brown AJP, Brown JD

Publication type: Article

Publication status: Published

Journal: Yeast

Year: 2003

Volume: 20

Issue: 15

Pages: 1235-1241

ISSN (print): 0749-503X

ISSN (electronic): 1097-0061

Publisher: John Wiley & Sons Ltd.


DOI: 10.1002/yea.1036

PubMed id: 14618561


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