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Lookup NU author(s): Christopher Blackwell,
Dr Jeremy Brown
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Protein A-tagging has become an important tool in characterization of protein-protein interactions in many systems, allowing purification of multicomponent complexes under native conditions. Here we provide a set of vectors that allow protein A-tagging in Candida albicans, through addition of the tag to open reading frames. These vectors were successfully used to generate stably tagged proteins that were functional, shown to be localized appropriately or assembled into complexes. These new vectors comprise a useful addition to the C. albicans molecular toolbox. Copyright © 2003 John Wiley & Sons, Ltd.
Author(s): Blackwell C, Russell CL, Argimon S, Brown AJP, Brown JD
Publication type: Article
Publication status: Published
ISSN (print): 0749-503X
ISSN (electronic): 1097-0061
Publisher: John Wiley & Sons Ltd.
PubMed id: 14618561
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